Composite

Part:BBa_K4229053:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)


otsB with RBS of pET-Duet-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

otsB was cloned out the genomic DNA of e.Coli. And was cloned inside of pET-Duet-1, which was given to us by the CIBBS Toolbox from the Uni Freiburg.

References

He Li et al.: Enhanced production of trehalose in Escherichia coli by homolougous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarty, JBB, Vol. 113 No. 2, 224-232, 2012